HPLC stands for High Performance Liquid Chromatography. It is a principle used in analytical chemistry to separate, identify, and quantify components in a mixture.

• HPLC uses a liquid mobile phase and a...read more

HPLC = High performance liquid chromatography

Hplc is a technical instrument that are used in two mixture of components are separation, qualitaty and identification with the help of mobile phase and s...read more

Here are principle of quality control instruments

1. Principle of High-Performance Liquid Chromatography

2. Principle of Gas Chromatography

3. Principle of UV Visible spectrophotometer

4. Principle of Infrared spectrophotometer

5. Principle of Karl Fischer

6. Principle of pH

7. Principle of potentiometer

8. Principle of polarimeter

9. Principle of Thin layer chromatography

10. Principle of Refractometer

1. Principle of HPLC:

   "HPLC is separation technique of the components of mixture by using solid stationary phase and liquid mobile phase."

For remaining instrument principles refer this site

The distribution of the analyte (sample) between a mobile phase (eluent) and a stationary phase is the basis of the HPLC separation principle (packing material of the column). The molecules travel through the stationary phase more slowly depending on the chemical makeup of the analyte. The duration of a sample's "on-column" time is determined by the specific intermolecular interactions between the sample's molecules and the packing material. As a result, different components of a sample elute at various periods. Thus, the sample ingredients are successfully separated.

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