Column and mobile phase selection is based on the analyte properties and separation goals.

• Consider the analyte's physicochemical properties such as polarity, molecular weight, and stability.

• Choose a c...read more

Choose bonded phases on high purity, low acidity silica for best peak shape. Select a C18 or C8 bonded phase first for good retention and resolution with typical acidic, basic and neutral samples.

Choose bonded phases on high purity, low acidity silica for best peak shape. Select a C18 or C8 bonded phase first for good retention and resolution with typical acidic, basic and neutral samples.

Columns selection is main role for the separation of

compounds in method development.

1)Compounds solubility to be check

2)pKa of the compound

Columns selection is main role for the separation of

compounds in method development.

1)Compounds solubility to be check

2)pKa of the compound