What is hplc and what is used for detector calibration?

Question

Accepted Answer

HPLC is a technique used in chemistry to separate, identify, and quantify components in a mixture. Detector calibration ensures accurate measurement.

HPLC stands for High-Performance Liquid Chromatography.
It is used to separate and analyze components in a liquid mixture.
HPLC utilizes a stationary phase and a mobile phase to separate the components based on their interactions.
The detector in HPLC measures the concentration of the separated components.
Detector calibration involves adjusting the detector response to ensure accurate measurement.
Common methods for HPLC detector calibration include using standard solutions of known concentrations.
Calibration curves are created by plotting the detector response against the known concentrations.
The calibration curve is then used to determine the concentration of unknown samples.
Examples of detectors used in HPLC include UV-Vis detectors, fluorescence detectors, and refractive index detectors.

Accepted Answer

High performance liquid chromatography ,checking the peak response minimum and maximum absorbance at different nanometer,202 to 209 maximum absorbance 202nm,240 to 249 minimum absorbance 245nm,268 to 275 maximum absorbance 272nm

Accepted Answer

It is separate the mixture of components is called HPLC.. caffeine

What is hplc and what is used for detector calibration?

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