Why fixation nessary For slide ader fir morphology

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Fixation is a critical step in preparing microscopic slides, especially for studying cell and tissue morphology. It serves several important purposes:

1. Preservation of Structure: Fixation prevents the degradation of cellular and tissue structures by halting enzymatic and autolytic processes. This ensures that the cells' morphology remains intact and reflects their natural state.

2. Stabilization of Cellular Components: Fixatives cross-link proteins and other cellular components, making them more stable and less likely to distort or degrade during subsequent staining or processing.

3. Prevention of Decomposition: Fixatives protect the specimen from microbial contamination and decay, which could compromise the slide's integrity.

4. Enhancement of Staining: Fixatives improve the affinity of

Accepted Answer

tissues preservation for pathology diagnosis

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The process of colouring of various components and parts of a tissue for purpose of clear and absolute differentiation through use of different dyes (strains) is called staining. The nature of dyes may be acidic, basic or neutral.

Usually the acidic dyes stain (colour) the cytoplasmic part and basic dyes colour the nuclear part. However, with a nuclear dye cytoplasm may also be stained. For the purpose of undergraduates usually general staining (single staining) is used, which may colour both nucleus and cytoplasm at the same time.

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Fixation isthe first step in tissue preservation for pathological diagnosis. Fixation arrests autolysis and putrefaction, coagulates soluble and structural proteins, fortifies tissues against the deleterious effects of subsequent processing, and facilitates staining and morphology

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Fix the specimen for purpose microscopy

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To fix the smear of specimen on the slide for microscopy

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For stable to all small particles in smear.

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Results for fragmented RNA

Why fixation nessary For slide ader fir morphology

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