1)How much percent of buffer used in mobile phase 2) Why we use sls in disso and how much %Allowed

Question

Accepted Answer

The percentage of buffer used in mobile phase and the use of SLS in disso and its allowed percentage.

The percentage of buffer used in the mobile phase depends on the specific experiment and the desired separation of compounds.
Buffer is used in the mobile phase to maintain the pH and ionic strength, which can affect the separation and stability of analytes.
The percentage of buffer can range from 5% to 50% or more, depending on the specific requirements.
Sodium lauryl sulfate (SLS) is commonly used in dissolution testing to enhance the solubility and release of poorly soluble drugs.
The allowed percentage of SLS in dissolution testing depends on regulatory guidelines and the specific drug being tested.
For example, the USP (United States Pharmacopeia) allows a maximum concentration of 0.1% SLS in dissolution testing for immediate-release dosage forms.
However, different guidelines may exist for different countries or specific drug products.

Accepted Answer

Buffer concentration in mobile phase should be between 10mM to 50mM

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