Explain HPLC principle anf instrumentation and working process

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Accepted Answer

HPLC is a chromatography technique used to separate compounds in a mixture based on their interactions with a stationary phase and a mobile phase.

HPLC principle involves passing a sample mixture through a column containing a stationary phase, where different compounds interact differently and are separated based on their affinity for the stationary phase.
HPLC instrumentation includes a pump to deliver the mobile phase, a sample injector, a column where separation occurs, a detector to measure the separated compounds, and a data analysis system.
The working process of HPLC involves injecting a sample into the system, passing it through the column under high pressure, detecting the separated compounds, and analyzing the data to determine the composition of the sample.
Example: HPLC is commonly used in pharmaceutical industry for quality control of drugs to ensure purity and potency.

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