Ion exchange chromatography principle, its types and type of exchange, pH of the offer for protein with pI 4 and pI 6

Question

Accepted Answer

Ion exchange chromatography separates proteins based on their net charge, with different types and exchange capacities. pH affects protein binding.

Ion exchange chromatography separates proteins based on their net charge
Types include cation exchange (positively charged proteins bind to negatively charged resin) and anion exchange (negatively charged proteins bind to positively charged resin)
Exchange capacity refers to the amount of charge that can be exchanged on the resin
pH of the buffer affects protein binding - proteins bind when the pH is away from their isoelectric point (pI)
For a protein with pI 4, it will bind more at pH above 4, and for pI 6, it will bind more at pH below 6

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