Difference between Normal phase and Reverse Phase

Question

Accepted Answer

Normal phase and Reverse phase are two types of chromatography techniques used for separation of molecules based on their polarity.

Normal phase chromatography uses a polar stationary phase and a non-polar mobile phase, while reverse phase chromatography uses a non-polar stationary phase and a polar mobile phase.
Normal phase is used for separation of polar compounds, while reverse phase is used for separation of non-polar compounds.
Examples of normal phase chromatography include silica gel chromatography, while examples of reverse phase chromatography include C18 column chromatography.
Normal phase chromatography is typically used for separation of small molecules, while reverse phase chromatography is used for separation of larger molecules such as proteins and peptides.

Accepted Answer

The main difference between normal phase and reverse p.chromatography is that normal phase chromatography has a very polar stationary phase and a non-polar m,phase whereas reverse phase chromatography has a non-polar stationary phase and a polar mobile phase .

Difference between Normal phase and Reverse Phase

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