How to start method development of unknown compound by HPLC for RS ?

Question

Accepted Answer

To start method development of unknown compound by HPLC for RS, one should consider various factors.

Identify the properties of the compound such as polarity, solubility, and stability
Choose the appropriate column and mobile phase based on the properties of the compound
Optimize the chromatographic conditions by adjusting the flow rate, gradient, and temperature
Validate the method by checking the specificity, linearity, accuracy, and precision
Compare the retention time and peak shape of the unknown compound with the reference standard
Repeat the analysis to ensure the reproducibility and reliability of the method

Accepted Answer

First check the solubility of compound then choose proper mobile phase.

Choose proper column and fix the flow rate, wavelength of compound. After result check the retention time and accuracy of the peak.

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