Who to prepare buffers,difference btwn np-hplc,rp-hplc, uses of potentiometric titrantions

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Preparing buffers, differences between NP-HPLC and RP-HPLC, and uses of potentiometric titrations.

Buffers are prepared by mixing a weak acid and its conjugate base or a weak base and its conjugate acid.
NP-HPLC separates non-polar compounds while RP-HPLC separates polar compounds.
Potentiometric titrations are used to determine the concentration of an unknown solution by measuring the potential difference between two electrodes.
Potentiometric titrations are commonly used in the pharmaceutical industry to determine the purity of drugs.
NP-HPLC is commonly used for the separation of lipids and fatty acids while RP-HPLC is used for the separation of amino acids and peptides.

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