Explain the theory and working principle of HPLC

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Accepted Answer

HPLC is a chromatography technique used to separate, identify, and quantify components in a mixture.

HPLC stands for High-Performance Liquid Chromatography.
It works on the principle of separating components based on their affinity towards the stationary and mobile phases.
The stationary phase is a solid or liquid packed in a column, while the mobile phase is a liquid that flows through the column.
The sample is injected into the mobile phase, and as it flows through the column, the components get separated based on their interaction with the stationary phase.
The separated components are detected by a detector and analyzed to identify and quantify them.
HPLC is widely used in pharmaceuticals, food and beverage, environmental analysis, and many other industries.

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