Tell me about HPLC technique

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Accepted Answer

HPLC is a technique used to separate, identify, and quantify components in a mixture based on their interaction with a stationary phase and a mobile phase.

HPLC stands for High-Performance Liquid Chromatography.
It is widely used in various industries such as pharmaceuticals, food and beverage, environmental analysis, and forensic science.
HPLC involves the use of a liquid mobile phase and a solid or liquid stationary phase.
The sample mixture is injected into the HPLC system, and the components are separated based on their affinity for the stationary phase.
Different types of HPLC columns, such as reverse phase, normal phase, ion exchange, and size exclusion, can be used depending on the analytes of interest.
HPLC can be used to analyze a wide range of compounds including small molecules, proteins, peptides, and nucleic acids.
It provides high sensitivity, resolution, and reproducibility in separating complex mixtures.
Detection techniques like UV-Vis spectroscopy, fluorescence, and mass spectrometry are commonly used in conjunction with HPLC for compound identification and quantification.
HPLC is a versatile and powerful analytical technique that plays a crucial role in research, quality control, and drug development.

Accepted Answer

High Performance Liquid Chromatography technique is to seperate the API analytes using Stationary phase which is column and Mobile phase. Quantifying them using detector.

Tell me about HPLC technique

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