Type of columns in hplc

Question

Accepted Answer

The type of columns in HPLC are typically packed with stationary phase material to separate compounds based on their interactions with the stationary phase and mobile phase.

Columns in HPLC can be classified based on the type of stationary phase material used, such as reversed-phase, normal-phase, ion-exchange, size-exclusion, and affinity columns.
Reversed-phase columns are the most commonly used in HPLC, where the stationary phase is non-polar and the mobile phase is polar.
Normal-phase columns have a polar stationary phase and a non-polar mobile phase.
Ion-exchange columns separate compounds based on their charge.
Size-exclusion columns separate compounds based on their size.
Affinity columns separate compounds based on specific interactions, such as antigen-antibody interactions.

Type of columns in hplc

Top Reliance Life Sciences Young Professional interview questions & answers

Popular interview questions of Young Professional

Top HR questions asked in Reliance Life Sciences Young Professional