How to Confermetri test in p. A

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If the result of HIV screening test is positive then confirmatory test is done.

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1st Confirmation done through UV light if APB broth or green colony on agar show light yellow flourished light then it is confirmed that is Pseudomonas. Or you can also performed by subculture the colony or broth on selective media( cetrimide or milk agar) by striking.

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My growth level in oppertunity best of lifestyle

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Change to my family situations

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My life change perpos

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presumptive colonies onto nutrients agar and incubator at 35c+/-1for 24+/-1hour perform gram stain appendix 3,uv fluorescence catalase test appendix 2oxidase test appendix 2on colonies purifie...read more

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presumptive colonies onto nutrients agar and incubator at 35c+/-1for 24+/-1hour perform gram stain appendix 3,uv fluorescence catalase test appendix 2oxidase test appendix 2on colonies purifie...read more

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Streak presumptive colonies onto nutrients agar and incubator at 35c+/-1for 24+/-1hour perform gram stain appendix 3,uv fluorescence catalase test appendix 2oxidase test appendix 2on colonies purified on natural agar nutrients agar isa non selective medium, which allows purification of the original colony. Culture on non selective media reduce the incidence of gram Variability and also fals positive or negative results in oxidase and catslase strike colonies from each nutrient agar plat in to two Pseudomonas agar base plate. Incubator one plate at 41.5c+/-1for 48+/-2 hours and 1at 4c+/-1for 48+/-2 hours. The presence of growth at 41.5c and none at 4c rules out the presence of Pseudomonas fluorescence which grows at 4°© Pseudomonas fluorescence has similar characteristics to Pseudomonas Aeruginosa in parallel to this test colonies from nutrient agar should also be transferred to milk agar and incubator at 35°©+\-1°c for 24+/-1hour.psedumonas Aeruginosa will cause a yellow green pigment to diffuse in to the milk agar. Colonies which fluorescence under uv light are gram negative, oxidase and catslase positive and give a yellow green Diffusible pigment on milk agar can be confirmed as Pseudomonas Aeruginosa

How to Confermetri test in p. A

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