Principle of HPLC and GC

Question

Accepted Answer

HPLC and GC are analytical techniques used to separate and analyze components in a mixture based on their interactions with a stationary phase and mobile phase.

HPLC stands for High Performance Liquid Chromatography, where the sample is dissolved in a liquid mobile phase and separated based on interactions with a stationary phase.
GC stands for Gas Chromatography, where the sample is vaporized and separated based on interactions with a stationary phase inside a column.
Both techniques are widely used in analytical chemistry for quantification and identification of compounds in complex mixtures.
HPLC is more suitable for analyzing compounds that are heat-sensitive or non-volatile, while GC is better for volatile compounds.
The principle behind both techniques is the differential partitioning of analytes between the stationary and mobile phases.

Principle of HPLC and GC

Popular interview questions of ADL Officer