What are the different types of chromatography and their principles?

Question

Accepted Answer

Chromatography is a technique used to separate and analyze mixtures based on their different interactions with a stationary phase and a mobile phase.

Types of chromatography include gas chromatography, liquid chromatography, ion exchange chromatography, affinity chromatography, and size exclusion chromatography.
Gas chromatography separates compounds based on their volatility and interaction with a stationary phase inside a column.
Liquid chromatography separates compounds based on their affinity for the stationary phase, such as reversed-phase or normal-phase chromatography.
Ion exchange chromatography separates compounds based on their charge interactions with the stationary phase.
Affinity chromatography separates compounds based on specific interactions, such as antigen-antibody interactions.
Size exclusion chromatography separates compounds based on their size and molecular weight.

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