What is Hplc Principle

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HPLC principle is a chromatographic technique used to separate, identify, and quantify components in a mixture based on their interaction with a stationary phase and a mobile phase.

HPLC stands for High-Performance Liquid Chromatography.
It involves the use of a stationary phase (typically a solid or liquid) and a mobile phase (liquid) to separate and analyze components in a sample.
The separation is based on the differential interaction of the components with the stationary phase and the mobile phase.
The stationary phase can be packed in a column or coated on a solid support, while the mobile phase is pumped through the column.
Components with stronger interactions with the stationary phase will elute later, while those with weaker interactions will elute earlier.
HPLC is widely used in various fields such as pharmaceuticals, environmental analysis, food and beverage testing, forensic science, and more.
For example, HPLC can be used to analyze the concentration of drugs in blood samples, detect pesticides in water samples, or determine the composition of food additives.

What is Hplc Principle

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