Calibration parameters of HPLC are used to ensure accurate and precise measurements of analytes.

• Calibration parameters include retention time, peak area, and peak height.

• Retention time is the time it ...read more

There are a total 9 parameters for HPLC calibration, these are as follows;

1. Flow Rate Accuracy

2. Injector Precision

3. Injector Linearity

4. Wavelength accuracy of the detector

5. Detector Linearity

6. Gradient Composition Accuracy

7. Carry Over

8. Temperature Accuracy

9. Drift and Noise

Calibration of HPLC is performed on six month basis or after any major failure or after maintenance. Acceptance Criteria - Wavelength maxima found should be between 273 ± 2 nm. Wavelength maxima found...read more

Flow rate: 1.0 ml/min. Wavelength For UV: 254 NM. Wavelength For PDA: 254 nm (2D data collection at 3.6 nm bandwidth) Injection volume: 0.0 ml.

Flow rate: 1.0 ml/min. Wavelength For UV: 254 NM. Wavelength For PDA: 254 nm (2D data collection at 3.6 nm bandwidth) Injection volume: 0.0 ml.

Flow rate: 1.0 ml/min. Wavelength For UV: 254 NM. Wavelength For PDA: 254 nm (2D data collection at 3.6 nm bandwidth) Injection volume: 0.0 ml.