Centrient Pharmaceuticals Supervisor Interview Questions and Answers

Environmental monitoring in aseptic areas involves regular testing for contaminants to ensure a sterile environment.

• Perform air sampling to check for viable and non-viable particles.

• Conduct surface monitoring to detect any microbial contamination.

• Analyze water samples for microbial growth.

• Set acceptance criteria based on regulatory guidelines and company standards.

• Different grades include Grade A, B, C, and D with varying levels of cleanliness.

• Acceptance criteria may include ...read more

Bacterial endotoxin test is performed to ensure water and products are free from harmful endotoxins.

• Perform the test using Limulus Amebocyte Lysate (LAL) assay

• Acceptance criteria for water is typically <0.25 EU/ml

• Acceptance criteria for products varies depending on the type of product and its intended use

• Ensure proper controls are in place to validate the test results

Performing pH and weighing calibration involves following specific procedures to ensure accuracy.

• For pH calibration, use buffer solutions of known pH values to calibrate the pH meter.

• For weighing calibration, use calibrated weights to check the accuracy of the balance.

• Follow the manufacturer's instructions for both pH meter and balance calibration.

• Regularly perform calibration checks to maintain accuracy.

• Document all calibration procedures and results for traceability.

• Train s...read more

We are using a variety of microbial cultures including bacteria, fungi, and yeast for different purposes.

• Bacteria cultures such as E. coli, Bacillus subtilis

• Fungi cultures like Aspergillus niger, Penicillium chrysogenum

• Yeast cultures such as Saccharomyces cerevisiae

Media can be made by combining specific ingredients in a sterile environment. Growth promotion test is used to ensure the media supports the growth of microorganisms.

• Media is made by combining ingredients like agar, peptones, and nutrients in a sterile environment.

• The ingredients are mixed, sterilized, poured into plates or tubes, and allowed to solidify.

• Growth promotion test is performed by inoculating the media with known microorganisms to ensure they grow as expected.

• If th...read more