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Naukri Interview Questions and Answers

Updated 5 Feb 2024
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Q1. How to perform environmental monitoring in aspetic area. What is their different grade and their acceptance criteria.

Ans.

Environmental monitoring in aseptic areas involves regular testing for contaminants to ensure a sterile environment.

  • Perform air sampling to check for viable and non-viable particles.

  • Conduct surface monitoring to detect any microbial contamination.

  • Analyze water samples for microbial growth.

  • Set acceptance criteria based on regulatory guidelines and company standards.

  • Different grades include Grade A, B, C, and D with varying levels of cleanliness.

  • Acceptance criteria may include ...read more

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Q2. How to perform bacterial endotoxin test and their acceptance criteria for water and product.

Ans.

Bacterial endotoxin test is performed to ensure water and products are free from harmful endotoxins.

  • Perform the test using Limulus Amebocyte Lysate (LAL) assay

  • Acceptance criteria for water is typically <0.25 EU/ml

  • Acceptance criteria for products varies depending on the type of product and its intended use

  • Ensure proper controls are in place to validate the test results

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Q3. How to perform ph and weighing calibration.

Ans.

Performing pH and weighing calibration involves following specific procedures to ensure accuracy.

  • For pH calibration, use buffer solutions of known pH values to calibrate the pH meter.

  • For weighing calibration, use calibrated weights to check the accuracy of the balance.

  • Follow the manufacturer's instructions for both pH meter and balance calibration.

  • Regularly perform calibration checks to maintain accuracy.

  • Document all calibration procedures and results for traceability.

  • Train s...read more

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Q4. What are microbial culture that you are using.

Ans.

We are using a variety of microbial cultures including bacteria, fungi, and yeast for different purposes.

  • Bacteria cultures such as E. coli, Bacillus subtilis

  • Fungi cultures like Aspergillus niger, Penicillium chrysogenum

  • Yeast cultures such as Saccharomyces cerevisiae

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Q5. How to make media. What is growth promotion test.

Ans.

Media can be made by combining specific ingredients in a sterile environment. Growth promotion test is used to ensure the media supports the growth of microorganisms.

  • Media is made by combining ingredients like agar, peptones, and nutrients in a sterile environment.

  • The ingredients are mixed, sterilized, poured into plates or tubes, and allowed to solidify.

  • Growth promotion test is performed by inoculating the media with known microorganisms to ensure they grow as expected.

  • If th...read more

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Q6. What is principal of autoclave.

Ans.

Autoclave is a device used to sterilize equipment and supplies by subjecting them to high pressure saturated steam.

  • Autoclave uses high pressure and high temperature steam to kill microorganisms on equipment and supplies.

  • It is commonly used in medical and laboratory settings to sterilize instruments and tools.

  • Autoclaves are essential for ensuring that equipment is free from harmful bacteria and viruses.

  • Proper maintenance and monitoring of autoclaves are necessary to ensure eff...read more

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