what is HPLC principle

Question

Accepted Answer

HPLC principle is based on the separation of compounds using a liquid mobile phase and a solid stationary phase.

HPLC stands for High Performance Liquid Chromatography.
It is a widely used analytical technique in chemistry and biochemistry.
The principle involves the separation of compounds based on their interaction with the stationary phase.
The mobile phase, usually a liquid solvent, carries the sample through the stationary phase.
Different compounds interact differently with the stationary phase, leading to their separation.
Retention time is used to identify and quantify the compounds.
HPLC can be used for various applications such as drug analysis, environmental monitoring, and food testing.

Accepted Answer

high(H) performance (p) liquid(L) chromatography (c). separation may result due to adsorption (or) partition (or) by both phenomenon depending upon the nature of adsorbent used on plates and solvents used for development.

what is HPLC principle

Top Biocon Limited QA QC Chemist interview questions & answers

Popular interview questions of QA QC Chemist

Top HR questions asked in Biocon Limited QA QC Chemist