What is assay by HPLC and ehy perform?

Question

Accepted Answer

Assay by HPLC is a technique used to separate, identify, and quantify components in a sample.

HPLC stands for High-Performance Liquid Chromatography.
It is commonly used in pharmaceutical and chemical industries for quality control purposes.
Assay by HPLC involves injecting a sample into a high-pressure liquid chromatography system, where the components are separated based on their interactions with the stationary phase.
The separated components are then detected and quantified using various detectors, such as UV-Vis or mass spectrometry.
This technique is performed to determine the purity, potency, and concentration of active pharmaceutical ingredients (APIs) in drug formulations.
It is also used to analyze impurities, degradation products, and related substances in pharmaceutical products.
Assay by HPLC helps ensure the quality and consistency of pharmaceutical products, as well as compliance with regulatory standards.
For example, in the analysis of a drug formulation, HPLC can be used to determine the concentration of the active ingredient and detect any impurities present.

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