Difference between Normal phase and reverse phase HPLC

Question

Accepted Answer

Normal phase and reverse phase HPLC differ in the polarity of the stationary and mobile phases.

Normal phase HPLC uses a polar stationary phase and a non-polar mobile phase.
Reverse phase HPLC uses a non-polar stationary phase and a polar mobile phase.
Normal phase is used for separating polar compounds while reverse phase is used for non-polar compounds.
Examples of normal phase solvents include hexane and heptane while examples of reverse phase solvents include water and methanol.

Accepted Answer

Normal phase stationary phase more than mobile phase

Reverse phase mobile phase more than stationary phase

Accepted Answer

The key differences between reverse phase and normal phase HPLC is that the reverse phase HPLC uses a nonpolar stationary phase and a polar mobile phase whereas the normal phase HPLC uses a poler mobile stationary phase and a less poler mobile phase

Accepted Answer

In normal phase stationery phase  is polar, mobila phase is non polar

Difference between Normal phase and reverse phase HPLC

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