Accutest Research Laboratories Research Scientist Interview Questions and Answers

Reaction conditions for chemical transformations

• Reaction temperature

• Reaction time

• Catalyst used

• Solvent used

• Concentration of reactants

• pH of reaction mixture

• Pressure

• Stirring rate

• Light exposure

• Oxidizing or reducing agents used

NMR peaks of solvents are important for identifying and quantifying compounds in solution.

• Different solvents have different NMR peaks due to their unique chemical structures

• Common solvents used in NMR spectroscopy include CDCl3, DMSO-d6, and acetone-d6

• The NMR peaks of solvents can interfere with the peaks of the compound being studied, so it is important to choose a solvent with minimal interference

• The chemical shift o...read more

I have handled various instruments in my research work.

• Microscopes for observing samples at high magnification

• Spectrophotometers for measuring the absorbance or emission of light by substances

• Centrifuges for separating components of a mixture based on density

• PCR machines for amplifying DNA sequences

• Electrophoresis equipment for separating DNA, RNA, or proteins based on size and charge

HPLC stands for High Performance Liquid Chromatography. It is a technique used to separate, identify, and quantify components in a mixture.

• HPLC works on the principle of differential partitioning of analytes between a stationary phase and a mobile phase.

• The stationary phase is a solid or liquid material packed into a column, while the mobile phase is a liquid solvent that carries the sample through the column.

• Analytes ...read more

Chloro and bromo pattern ratio refers to the ratio of the number of chloro and bromo substituents on a molecule.

• The chloro and bromo pattern ratio is calculated by dividing the number of chloro substituents by the number of bromo substituents.

• For example, if a molecule has 2 chloro substituents and 1 bromo substituent, the chloro and bromo pattern ratio would be 2:1.

• The chloro and bromo pattern ratio can be used to det...read more